How to change source, gas and other default acquisition method parameters in Analyst® software?

For research use only. Not for use in diagnostic procedures.

Answer

When a new method is opened in Analyst® software, the source and gas parameters, including the declustering potential (DP), the collision energy (CE) and the collision cell exit potential (CXP), are populated based on the software's default parameters. These source and gas default parameters are the default values for any new acquisition method in both the Tune and Calibrate mode (for manual and automatic optimization) and the Acquire mode (for LC/MS acquisition methods). The source parameter default values are stored in instrument parameter settings, and these can be edited and changed so that a desired value is populated in the method by default instead.

Prior to making changes to the source and gas default parameters, It is important to take a screen shot of the settings and save it as a backup. Do not change any other parameters in this window and contact SCIEX Technical Support with any additional questions.

Before changing any default parameters, ensure all acquisition methods and other windows are saved and closed in the Analyst software. To access the default parameter settings, select the Configure mode, and then navigate to the Tools menu. Select Settings and then Parameter settings. In the Scan Category field, select the desired experiment from the drop-down menu. Double-click on the source parameter to be changed, and then enter the default value in the Access Default field. Select OK to close and save the new default parameter. After changing all the desired source and gas parameter defaults, select OK on the parameter settings edit window.



Note: Make sure to change source values consistently across all scan categories of the same polarity. For example, if the value for TEM is changed to 300°C for a Q1+ scan, also ensure that the same value is used for Q3+ and MS/MS+ scan modes. This is especially important when performing automatic compound optimization when different scan modes are used sequentially.

Please use extreme caution and do not change other instrument parameters outside of the source and gas parameters. Even for changes to source parameters, its is best to consult the SCIEX support team.