知识库文章

Canned Calculation for ICH Guideline M10 – 3.2.2 Specificity Available for SCIEX OS Software

This article provides information on the custom calculations required for processing data according to the guidance found in ICH guideline M10 – 3.2.2. The guidance requires that it is demonstrated that no significant response attributable to interfering components compared to the analyte or IS response in the blank and double blank samples, respectively, is observed. These calculations were created to provide a ready-made solution for determining which samples will need to be re-injected in pesticide reports.

Considerations when Creating a Library-Free Proteomics Experiment without the OneOmics™ Suite

Library-free proteomics experiments require highly reproducible sample-handling protocols and data analysis and can be done directly in DIA-NN software without any other SCIEX software or ion libraries. 

How Does the Channel Alignment Function on a SCIEX TripleTOF® System?

The purpose of channel alignment is to re-align the signals from each detector/TDC channel in time on a TripleTOF® system. Channel 2 is used as the reference channel, and a simple linear transformation is applied to the other three channels to align them to the refrence.

Troubleshooting the ZenoTOF 7600 System: In-Run Calibration Failures

If a calibration failure occurs while acquiring data on the ZenoTOF 7600 system, then certain measures can be followed to mitigate this issue. The troubleshooting guide provided in this article is a step-by-step path for resolving any problems with in-run calibrations. To begin troubleshooting in-run calibration failures, users should open the calibration report in the Analytics workspace of SCIEX OS software to identify the reasons behind the failure.

What Fittings Are Needed To Add a Waters M-Class LC to My ZenoTOF 7600 System?

This article explains which fittings are needed to add a Waters M-Class LC to a ZenoTOF 7600 system and includes part numbers and pictures of tubing and trap loop connections.

How To Reset a QTOF System Mass Spectrometer

Power cycling a QTOF mass spectrometer can help resolve communication issues. The The TripleTOF® system, the X500 QTOF system, and the ZenoTOF system instruments (X500-, 5600-, 6600- and 7600-series instruments) are all reset in the same way. This article describes the reset procedure for these instruments.

Why Is There No MRMHR Scan Type when Using the Method Wizard for a TripleTOF® LC-MS/MS System?

This article describes the MRMHR workflow for a TripleTOF® LC-MS/MS System using the Method Wizard in Analyst® TF or SCIEX OS Software. MRMHR is not a scan type. It is a description for a set of looped experiments, which can be used for a targeted approach. The looped experiments include a TOF-MS experiment followed by dedicated product ion experiments either in high-resolution or high-sensitivity mode.

Saving Consecutive Batches for the Echo® MS System to the Same Data File Impairs Peak Splitting, Automatic Processing

For data acquired with the Echo® MS system, peak splitting is performed after acquisition is complete. If a subsequent batch is acquiring data to a file while the system is splitting peaks written to that file during the previous acquisition, then a resource conflict occurs.

Is Digital PicoView® Source Windows 10 compatible?

Digital PicoView® Source is a nano ion source from New Objectives that is Windows 10 compatible and can be used with many SCIEX mass spectrometers.

How Is the ITC Parameter Accessed in Analyst® TF Software?

Changing the ion transmission control (ITC) parameter in Analyst® TF Software helps to assess if the QJet® ion guide on a Triple TOF® system needs to be cleaned. This article explains how to access the ITC parameter within the Analyst software.

How Does the Quadrupole Transmission Function in the TripleTOF® Systems Work?

For TOFMS, all ions pass through Q1 in RF mode. In general, Q1 and Q2 perform "hops" over the mass range, ensuring an appropriate envelope of masses is passed into the TOF analyzer. The general explanation found here for the QSTAR® systems can also be used to explain TripleTOF® systems as well.

Which Calibration Standards Can Be Used for Intact Protein Analysis on TripleTOF® and X500 Systemss?

An APCI calibrant solution for the SCIEX X500B System and a mAb standard from Waters were compared. Results show that the mass to charge ratio for the calibrant solutions remain constant regardless of the charge state. Secondly, for readings over 500 m/z, the calibration is highly linear for the X500B system.

Can a Precursor-ion IDA Experiment Be Performed on a SCIEX TripleTOF System?

None of the SCIEX TOF instruments, 5600, 5600+, 6600, X500R, or X500B, can do a precursor ion IDA experiment.