知识库文章

How can I enable the MSServiceLog Script in Analyst® software to record readbacks from a mass spectrometer?

By default, readbacks from a mass spectrometer are recorded in the MS Service log file. Use the MSServiceLog script to turn off the recording of the readbacks or to start recording the readbacks from the instrument in the MS Service log file. The MSServiceLog script is only applicable to the SCIEX 3500, 4500, 5500, 5500+, 6500, and 6500+ systems. The MSServiceLog script can be used without an active hardware profile but any changes made to the MS Service log settings take effect only after the hardware profile is reactivated.

How to perform Positive charging test using Sciex OS® on 7500 instruments

How to perform Positive charging test using Sciex OS® on 7500 instruments

How to perform Positive charging test using Sciex OS® on Blueline instruments

How to perform Positive charging test using Sciex OS® on Blueline instruments

How does increasing column temperature affect LC methods?

Increasing the column temperature can affect liquid chromatography (LC) parameters positively by decreasing column pressure, reducing retention times, and lowering the overall analysis time. Fluctuation in column temperature can result in unstable retention times and peak shapes.

How to Reduce the Column Pressure during Liquid Chromatography?

When a new liquid chromatography (LC) method is developed, the pressure might be too high and close to the maximum pressure allowed by the hardware. It is important to address this issue before validating the method.

What is matrix effect and how is it quantified?

The loss of analyte signal due to presence of matrix in sample is called matrix effect in analytical chemistry. Here the quantitation of matrix effect is also explained.

How can I quantify efficiency of my extraction method in SCIEX OS software?

Extraction efficiency is the percentage of analyte recovered through the extraction process, from the start of extraction to the final step of sample preparation prior to LC/MS analysis. This article explains how to quantify the extraction efficiency in SCIEX OS software.

What are the suggested time intervals when using a diverter valve?

Efficient use of diverter valve will keep the mass spectrometer source and ion optics from unnecessary and excessive contamination. This article provides guidance on how to set an appropriate time range for use of a diverter valve during an acquisition method.

Which mass spectrometers and ion sources are supported by SCIEX OS software?

If a new flow rate is desired for a LC/MS experiment, a different ion source may be needed for your SCIEX mass spectrometer. Included here is a list of current mass spectrometers and ion sources that are supported by the most recently released of SCIEX OS software, version 3.3.

What is the best way to flush a liquid chromatography system to remove contamination?

Contamination of a liquid chromatography (LC) system usually requires flushing the system with an organic solvent to remove unwanted background. Here an effective way to flush the entire LC system is discussed.

Why do extracted samples and quality controls have lower signal compared to neat standards?

When a sample or a spiked quality control is extracted, the signal is usually lower than that of the corresponding standard made in pure solvents.

Removing an ESI probe to change the capillary on a SCIEX mass spectrometer

Capillaries can get blocked and will need to be replaced from time to time to maintain high quality spray from an electrospray ionization (ESI) probe. This article provides a link to a YouTube video that shows how to remove an ESI probe to change the capillary on a SCIEX mass spectrometer.

DIP Switch Positions when Connecting Agilent LC Systems to SCIEX Mass Spectrometers

When connecting Agilent LC Systems to a SCIEX mass spectrometer, the dual-in line packet (DIP) switches on the Agilent LC systems should be in the down (off) position, except for switches 7 and 8 which must be in the up position for the pump and detector.

Diverter Valve Would Not Work While Using Delay Time in Analyst® Software

The diverter valve will not work properly when using Delay Time in the acquisition method of Analyst® Software due to a mismatch between diverter valve timing and MS acquisition timing. This results in the diverter valve timing not aligning with the MS acquisition time.

Adding a New Fragmentation Rule for EAD Data from a ZenoTOF 7600 System to the Mascot Server

The steps for adding Mascot server fragmentation rules for an electron activated dissociation (EAD) cell on a ZenoTOF 7600 system are discussed in this article, as well as how to set up these rules in the Mascot Daemon client software version 2.6 or 2.8.

How Do I Convert Acquisition Methods Created in Analyst® Software for Use in SCIEX OS Software?

Users can convert existing acquisition methods created in Analyst® software for use in SCIEX OS software by using the Convert File option in the Open menu in the MS Method tile. Only the MS portion of the method will be converted; there is no conversion function for the LC portion of the method.

What Are the Specifications for Installing SCIEX OS Software 2.2 on an Acquisition PC?

To install SCIEX OS software version 2.2 on an acquisition workstation, the PC needs to meet specific requirements. The following PCs are supported: 1) SCIEX workstation or workstation+ and 2) SCIEX Alpha workstation 2020.

How to Interface MultiQuant™ Software with a LIMS System via a LIMS Report Template

Oftentimes MultiQuant™ software results table data needs to be exported and then interfaced with a LIMS system. This article explains how to indirectly transfer data from results tables created in MultiQuant software to a LIMS system via a report template. The report template creates an output file in CSV format that can then be interfaced with a LIMS system.

Obtaining a Smaller Volume Divert Valve for a 4500, 5500 and 6500 LCMS Systems

To obtain a smaller volume divert valve for a 4500, 5500 or 6500 LCMS system, a new part must be ordered from Valco, part number of C52-2006I.

How to Determine if the Acquisition PC Has a Bad Ethernet Card?

This article summarizes some issues that might contribute or confirm a bad network card.

What Is the Settling Time in an Analyst® Software Acquisition Method?

In Analyst® software, the settling time is defined as the time that a mass spectrometer pauses after finishing a cycle until it begins the next cycle. The instrument electronics require a settling time in order to function successfully.

Longer Power Cycle May Help To Resume Instrument Connectivity after Power Failure

Even after resetting the mass spectrometer (by following the power-cycling procedure), the instrument would not come back online after a power failure. On some occasions, the mass spectrometer must be completely powered down for at least 1 min in order for the circuits to discharge and fully release any faulty information.

Where Can the Software for StatusScope® Remote Monitoring Service Be Accessed?

To obtain access to the software download for StatusScope® Remote Monitoring service, users who are under warranty or who have purchased the service can navigate to statusscope.absciex.com and select the link to the software download.

How to adjust, save and export the table settings in an Analyst Quantitation Table

Instructions how to modify (choose and adjust the visible columns, change the significant figures and notation), save and export an Analyst Quantitation Table.

Ordering Foam Applicators for Cleaning a IonDrive™ QJet Ion Guide

The IonDrive™ QJet ion guide can be cleaned with an anti-static foam swab (part number 1017396 or 1017398).

Using the 25 Micron ESI Electrode with the DuoSpray™ Source?

A 25 micron ESI electrode can be used with the IonDrive™ Turbo V source and the DuoSpray™ source.

Using Ammonium Fluoride (AMF) in an HPLC Solvent

Ammonium fluoride can be used in LC/MS applications as a mobile phase additive. It is recommended that only small concentrations as possible be used (around 0.2 mM).

What Are the Reasons for Hybrid Electrodes Probe Blockage?

Common issue reported with hybrid electrodes probes.

Integrated Syringe Error Message Recieved while Using Manual Tune Mode in Analyst® Software

While running Analyst® software in Manual Tune mode during compound optimization, an error message is received: "The current sample cannot be run because the syringe pump has stalled. The syringe plunger may be stalled in the syringe barrel." To resolve this issue, move the syringe holder back and clear error. If this doesn't work, replace the syringe, or if still needed, reset the MS.

What Files Must Be Backed Up before an Analyst Software Reinstall?

Back up the API Instrument project folder in the Analyst Data folder prior to any reinstall or upgrade of the Analyst software. To upgrade an older version of Analyst, remove and back up the Analyst Data folder and any remaining short cuts on your workstation. Reboot the PC and install the new version of the software.

Injection volume must be a positive value

If the Regional Settings in an Acquisition Computer with Analyst® Software is not set to US English , this can course the Error message: "Batch not submitted-Injection volume must be a positive value" during submitting a Batch.

How Do You Set Up for Manual Tuning Using Tee Infusion?

Before setting up your manual tuning method for source/gas optimization, your MS instrument must be set up for continuous infusion with high LC flow.

How do I tell if the source exhaust is blocked or flooded? What is the cause and how do I fix it?

Procedures to diagnose exhaust issues.

What is my Source Exhaust and how does it work?

Description of the Source Exhaust components and common requirements to maintain optimal performance

Power cycling the PC, HPLC, and MS to solve communication problems

A power cycle can be performed on LCMS components to solve communication problems.

How to wire SCIEX AUXIO cable for use with a Waters Acquity HPLC System

Diagram of how to wire AUXIO connections for the Acquity HPLC including the Stop Flow on instrument error

Setup of Diverter Valve

How to setup a diverter valve, including plumbing and Analyst software setup.

Tuning 4500,5500 and 6500 using Instrument Optimization

Walk through of instrument optimization

Venting and Pumping Down the 5500 Series Mass Spectrometer

How to remove the vacuum and pump the machine back down afterwards

Submitting and Starting Sample Batch in Analyst® Software

How to start acquiring data using Analyst software

Create Acquisition Method with Agilent HPLC in Analyst® Software

Walk Through of Analyst acquisition method (.dam) creation when a Agilent HPLC is employed.

Create Acquisition Method with Shimadzu HPLC in Analyst® Software

Walk through of creating an Analyst acquisition method (.dam) when a Shimadzu HPLC is employed.