知识库文章

List of CE Capillaries with Pre-burned Detection Windows

CE capillaries with pre-burned detection windows are available for purchase; the dimensions and coating information for these products are listed here.

Calibration Correction Factor (CCF) for Laser-Induced Fluorescence (LIF) Calibration

The Calibration Correction Factor (CCF) for laser-induced fluorescence (LIF) calibration is the mathematical relationship between the fluorescence from a known concentration of the analyte and the associated signal detected by the instrument. An explanation of how it is calculated is provided in this article.

Plotting 32Karat Software Data Files that Have Been Exported as ASCII Files into Excel

To plot 32Karat software data into a graph/electropherogram format, open the ASCII file in Excel and then format the data as described.

Understanding and optimizing signal-to-noise calculation in the 32Karat software

This document provides an overview of the S/N calculation and optimization in the 32Karat software.

Exporting reports and data graphic from the 32Karat method

Procedure to export reports (e.g. % area report, etc.) and data graphics (electropherograms with annotations) from the method.

Coolant leak troubleshooting tips

The document provides troubleshooting steps for coolant leak issues (to determine where the leak is coming from).

Exporting PDA data from 32Karat into ASCII or AIA (.CDF) files

Procedure to export CE data files from the 32Karat software.

Explanation of 32 Karat License Key Program and User License Types

32 Karat software requires a hardware license key to launch, and on older versions, a similar software license key is installed from a floppy disk.

Overview of the 32 Karat Software User Privileges

An overview is provided of each user privilege in the 32 Karat software that can be assigned or removed from the user.

Where Can LIF Emission and UV Bandpass Filters Be Ordered?

UV bandpass filters used with the fixed wavelength UV detectors or emission filters for the laser induced fluorescence (LIF) detector can be ordered from common optics companies such as Andover Optics or Newport Optics.

Using 32 Karat Software with a Corporate Network or Domain

32 Karat software can be used with a company network 1) to control user logins and permissions via a Windows operating system (OS) network domain controller and 2) to allow access to network printers and hard drives.

Common Issues when Using 32 Karat Software for Peak Integration

Peak integration with the 32 Karat software can be improved using the suggestions found here.

Improving Peak Resolution when Using Capillary Electrophoresis

Basic tips are presented here for improving the overall resolution of capillary electrophoresis (CE) separations.

Charge Heterogeneity Buffer Vial Plate Map for Tech Bulletin IB-17031A

The plate map used in the SCIEX charge heterogeneity capillary zonal electrophoresis (CZE) assay will help guide users in setting up the method in the tech bulletin "Analysis of Monoclonal Antibody Charge Variants by Capillary Zone Electrophoresis" (Beckman Coulter IB-17031A).

PA800 How To - Changing Coolant Tubing

Changing Coolant Tubing on PA800 System

PA800 How To - Trimming a Capillary

Trimming a Capillary

PA800 How To - Installing the LIF Aperture and Probe Stabilizer

Installing the LIF Aperture and Probe Stabilizer

PA800 How To - Cartridge Removal

Video demonstration of Cartridge Removal on the PA800 system

PA800 How To - Capillary Removal

Capillary Removal

PA800 How To - Capillary Storage

Capillary Storage Details for PA800 System

PA800 How To - Installing the Cartridge Aperture

Procedure for Installing the Cartridge Aperture on the PA800 system

PA800 How To - Replacing the Ejector and Electrode

Replacing the Ejector and Electrode

PA800 How To - Uninstalling the LIF Detector

Uninstalling the LIF Detector

PA800 How To - Removing the Ejector and Electrode

Removing the Ejector and Electrode

PA800 How To - Installing the LIF Detector

Installing the LIF Detector

PA800 How To - Cartridge Removal Rebuild Reinstallation

Procedure for Cartridge Removal Rebuild Reinstallation on PA800 System

PA800 How To - Changing a Detector

Changing a Detector