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Why do extracted samples and quality controls have lower signal compared to neat standards?

When a sample or a spiked quality control is extracted, the signal is usually lower than that of the corresponding standard made in pure solvents.

Proteases such as trypsin and beta galactosidase sold by SCIEX have no certificates of analysis (CoA)

Certificates of analysis (CoA) are available for various reagents; however for proteases such as trypsin and beta galactosidase that are sold by SCIEX, no CoA is available.

10/05/2023    Standards and Reagents

To Prevent Contamination, Use Formic Acid Ampules

To prevent your samples from becoming contaminated, use formic acid from small volume ampules rather from a large bottle. Impurities can accrue when taking aliquots of formic acid multiple times from the same bottle, and these impurities can impact positive ion analysis especially.

12/28/2018    Standards and Reagents

What Are the Peptide Sequences in the Six-Peptide Mixture (PN 4465940)?

The six-peptide mixture (part number 4465940) is used for the calibration of mass spectrometers. The kit contains the following six peptides and their mono-isotopic masses: bradykinin (2-9 clip) (904.4681), angiotensin I (1296.6853), glu1-fibrinopeptide (1570.6774), ACTH (1-17 clip) (2093.0867), ACTH (18-39 clip) (2465.1989), and ACTH (7-38 clip) (3657.9294).

Which Amino Acids in the PepCalMix Are Labeled?

The PepCal Mix (P/N 5045759) contains 20 heavy-labeled peptides, with the heavy isotopes incorporated in the amino acids, K and R.

Where Can I Find Instructions for the Protein Preparation Kit?

Protein preparation instructions can be found on the SCIEX website at https://sciex.com/Documents/tech%20notes/Protein-Preparation-Kit-Protocol.pdf or https://sciex.com/products/standards-and-reagents/automated-protein-digestion-solution.

Where Can I find Safety Data Sheets (MSDS) or Certificates of Analysis (CoA)?

To find the Safety Data Sheets and Certificate of Analysis for Sciex reagents, navigate to https://sciex.com/tech-regulatory?safetyTerm=4406127&safetyLanguage=English-United+States

12/11/2017    Standards and Reagents

Acetic Acid Is Needed To Prepare the PepCalMix Peptides in the SWATH® Acquisition Performance Kit

This article describes the experimental issues when formic acid is used to prepare the PepCalMix for the SWATH® Acquisition Performance Kit instead of 5% acetic acid.