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Adjust the regional language in LibraryView™ software to correct the SCIEX L:icense Notification screen

This article describes how to change the regional language settings within LibraryView™ and SCIEX OS Software. This prevents the license notification screen from popping up.

03/15/2024    SCIEX OS

Canned Calculation for SANTE Guideline 12682_2019 - C18 Available for SCIEX OS Software

This article provides information on the custom calculations required for processing data according to the guidance found in SANTE 12682_2019 - C18. The guidance section deals with the requirements to perform calibration by interpolation.

02/28/2024    SCIEX OS

Canned Calculation for ICH Guideline M10 – 3.2.2 Specificity Available for SCIEX OS Software

This article provides information on the custom calculations required for processing data according to the guidance found in ICH guideline M10 – 3.2.2. The guidance requires that it is demonstrated that no significant response attributable to interfering components compared to the analyte or IS response in the blank and double blank samples, respectively, is observed. These calculations were created to provide a ready-made solution for determining which samples will need to be re-injected in pesticide reports.

Create fast optimization acquisition methods for Q0D mapping in SCIEX OS software

With the Q0D mapping calculation sheet you can easily create acquisition methods for the optimization of Q0D values on column with matrix.

01/31/2024    SCIEX OS

Canned Calculation for SANTE Guideline 12830 2020 – Residual Plot Available for SCIEX OS Software

This article provides information on the custom calculations required for processing data according to the guidance found in SANTE Guideline 12830 2020 – Residual Plot. The guidance recommends that the suitability of the chosen calibration function is demonstrated with a residual plot. These calculations were created to provide a ready-made solution for the visual inspection of the residuals and can be combined with a specific report.

01/30/2024    SCIEX OS

Canned Calculation for ICH Guideline M10 – 3.2.1 Selectivity Available for SCIEX OS Software

This article provides information on the custom calculations required for processing data according to the guidance found in ICH guideline M10 – 3.2.1. The guidance requires that it is demonstrated that no significant response attributable to interfering components is observed at the retention time(s) of the analyte or the IS in the blank samples. These calculations were created to provide a ready-made solution for flagging problematic samples/components.

01/30/2024    Pharma CRO | SCIEX OS

Canned Calculation for ICH Guideline M10 – 3.2.4/3.3.2 Calibration Standard Accuracy Available for SCIEX OS Software

This article provides information on the custom calculations required for processing data according to the guidance found in ICH guideline M10 – 3.2.4/3.3.2. The guidance requires that the accuracy of the back-calculated concentrations of each calibration standard should be within ±20% of the nominal concentration at the LLOQ and within ±15% at all the other levels. At least 75% of the calibration standards with a minimum of 6 calibration standard levels should meet the above criteria. These calculations were created to provide a ready-made solution for flagging problematic samples/components.

01/30/2024    Pharma CRO | SCIEX OS

Canned Calculation for SANTE Guideline 12682_2019 - C15 Available for SCIEX OS Software

This article provides information on the custom calculations required for processing data according to the guidance found in SANTE 12682_2019 - C15. The guidance requires that without a bracketing calibration the determination system has been shown to be free from significant drift, e.g. by monitoring the response of an internal standard. These calculations were created to provide a ready-made solution for evaluating a possible drift in the internal standard response.

01/30/2024    SCIEX OS

Generating a Service Package in Sciex OS Software

To generate a service package in Sciex OS software that contains the event logs, select Configuration, About, and Generate a Support Package.

01/15/2024    SCIEX OS

How to generate reports in CSV format with SCIEX OS if no comma-separated report style is visible

If no comma-separated report style is visible, you must change the extension of the TXT file to CSV when saving the file.

11/14/2023    SCIEX OS

Molecule Profiler software 1.3 supports the entire sequence of an antibody drug conjugate (ADC) in a dedicated workflow

Molecule Profiler software version 1.3 supports a dedicated workflow for antibody drug conjugates (ADCs). The entire antibody sequence can be copied into the software for processing.

How To Report Out Regression Types in Reporter Template Suite 3.2

To report out the regression type used in a curve fit, a combination of IF, ELSEIF, and ELSE tags are required in the report template in Reporter Template Suite 3.2.

Inputting known concentrations of analytes into a batch in SCIEX OS software

In SCIEX OS software it is possible to input known concentrations of analytes and internal standards for Quality Control and Standard sample types. This prevents having to add the values directly to the results table and saves time when analyzing data.

10/20/2023    SCIEX OS

Processing parameters for an antibody drug conjugate (ADC) Workflow in Molecule Profiler software 1.3

The antibody drug conjugate (ADC) workflow in Molecular Profiler software 1.3 helps to identify and further characterize cytotoxic drugs, their breakdown products, and the linker connected to the entire antibody sequence. This article describes how to set up the processing parameters for the ADC workflow.

What Are the Different Data File Types in Analyst® and SCIEX OS Software?

In the data sub-project within a project folder in Analyst® or SCIEX OS Software, there can be a multitude of file types associated with one set of raw data. The file types and the software they are associated with are listed in this article.

10/20/2023    SCIEX OS | Analyst Software

Optimization Report (XPS File) in SCIEX OS Software Does Not Display Graphs

After completing a multiple reaction monitoring (MRM) infusion in SCIEX OS software, the optimization report (.xps file) does not have any graphs displayed even though the graphs appeared in the software after the optimization was completed. This is due to a Windows update (update for Microsoft Windows KB5020866) that restricts .xps files.

10/20/2023    SCIEX OS

How can I quantify efficiency of my extraction method in SCIEX OS software?

Extraction efficiency is the percentage of analyte recovered through the extraction process, from the start of extraction to the final step of sample preparation prior to LC/MS analysis. This article explains how to quantify the extraction efficiency in SCIEX OS software.

10/19/2023    SCIEX OS | Mass Spectrometers

What are the suggested time intervals when using a diverter valve?

Efficient use of diverter valve will keep the mass spectrometer source and ion optics from unnecessary and excessive contamination. This article provides guidance on how to set an appropriate time range for use of a diverter valve during an acquisition method.

Which mass spectrometers and ion sources are supported by SCIEX OS software?

If a new flow rate is desired for a LC/MS experiment, a different ion source may be needed for your SCIEX mass spectrometer. Included here is a list of current mass spectrometers and ion sources that are supported by the most recently released of SCIEX OS software, version 3.3.

10/18/2023    SCIEX OS | Mass Spectrometers

Common installation issues when upgrading SCIEX OS software

Some of the most common issues encountered while upgrading the SCIEX OS software and how to prevent them are listed here. Please consult the SCIEX OS software installation guide found in the installer package for step-by-step instructions for correctly upgrading the software.

10/14/2023    SCIEX OS

Common Installation Errors for SCIEX OS Software

This article describes the common installation issues that occur when installing SCIEX OS software for the first time. To install SCIEX OS software successfully, the Installation Guide should be consulted and each section should be read prior to installing the software.

10/13/2023    SCIEX OS

How To Change the Number of Available Rows in the Batch Workspace in SCIEX OS Software

In SCIEX OS software, it is possible to change the number of available rows in the batch. To do this, select the View button in the top right of the batch workspace.

10/13/2023    SCIEX OS

How To Utilize the Quick Batch Option in SCIEX OS Software

A new option for creating batches quickly was introduced in SCIEX OS software version 3.1.6 and is called Quick Batch. To utilize Quick Batch, an icon in the upper right corner of the Batch screen will need to be selected.

10/13/2023    SCIEX OS

Can two analytes have the same transitions in an MRM method in SCIEX OS software?

Occasionally two different analytes in the same MRM method in SCIEX OS software may have identical Q1 and Q3 masses for their respective MRM transitions. The question is if this could cause problem in quantitation and what should the user do to avoid errors.

10/13/2023    SCIEX OS

Can SCIEX OS software be used for acquisition on all SCIEX instruments?

To determine if a particular version of SCIEX OS software supports acquisition on a SCIEX instrument, please consult the installation guide for that version of the software. SCIEX OS software does not support acquisition on legacy instruments such as the API™ 3200 and 4000 systems.

10/13/2023    SCIEX OS

How can I set up contact closure in SCIEX OS software for my SCIEX instrument?

To set up contact closure for a SCIEX mass spectrometer to communicate with an external LC system, the contact closure check box must be selected in the instrument's hardware profile in SCIEX OS software. SCIEX OS software 3.1.6 supports many of the same legacy instruments that were controlled by Analyst® software even if contact closure is required for a third-party vendor LC system.

10/09/2023    SCIEX OS

Adding MS/MS information to the MRMHR processing method's Components tab in SCIEX OS software

To add the MS/MS information to a MRMHR processing method in SCIEX OS software, a compound name, a unique name for the fragment ion, and the fragment mass information are required in the table under the Components tab.

10/09/2023    SCIEX OS

How to upgrade your SCIEX mass spectrometer's firmware after upgrading the SCIEX OS software

After upgrading SCIEX OS software on an acquisition PC, the instrument firmware also needs to be upgraded. To do this, follow the instructions in the SCIEX OS software installation guide in the sections MS FW Updater Utility and Mass Spectrometer Firmware Versions.

10/08/2023    SCIEX OS

How To Ramp Parameters in SCIEX OS Software during Compound Optimization

When using SCIEX OS software to optimize compounds that are being infused using a syringe pump, it is possible to ramp individual compound parameters such as collision energy (CE), entrance potential (EP), and collision cell exit potential (CXP), and Q0 dissociation (Q0D). This process may be preferred to guided MRM optimization in the event that a more manual and controlled optimization is desired.

10/06/2023    SCIEX OS

What version of SCIEX OS software includes a tile for Biologics Explorer software?

A tile for Biologics Explorer (BE) software appears in SCIEX OS versions 3.1.5 and higher. The BE software can be launched from this tile, but the BE software needs to be installed from a separate installer as described in this article.

10/06/2023    Academia Omics | Pharma CRO | SCIEX OS

Impermissible Symbols for Root Directory Paths and Project Names in SCIEX OS Software

There are some symbols which cannot be used when naming a root directory path or a project in SCIEX OS software. They are detailed in this article.

10/06/2023    SCIEX OS

How To Change the Instrument Idle Time In SCIEX OS Software

After a set time (the instrument idle time) has elapsed, the system will go into Standby state – whereby the LC pumps, column oven and some mass spectrometer voltages are turned off. To change the instrument idle time in SCIEX OS software, navigate to the Configuration pane, and select the Queue tab. The instrument idle time can be changed by typing the desired time into the field.

09/22/2023    SCIEX OS

How Can I Transfer an Acquisition Method from Analyst® to SCIEX OS Software?

To transfer or import an acquisition method (*.dam file) created in Analyst® software into SCIEX OS software, open the MS Method tile and then select Convert File. Only the MS experiment will be imported into SCIEX OS software. The LC method from Analyst software will need to be entered manually.

06/06/2023    SCIEX OS | Analyst Software

How to use Mixed Mode in SCIEX OS

Explains the different Security Modes and how to use them. More details can be found in SCIEX OS Laboratory Directors Guide

02/07/2023    SCIEX OS

Diverter Valve Would Not Work While Using Delay Time in Analyst® Software

The diverter valve will not work properly when using Delay Time in the acquisition method of Analyst® Software due to a mismatch between diverter valve timing and MS acquisition timing. This results in the diverter valve timing not aligning with the MS acquisition time.

How Can I Adjust the Dynamic CE Table in SCIEX OS Software?

Sometimes the dynamic collision energy (CE) for peptides needs to be adjusted to get better fragmentation of the precursor ion. This article describes how to adjust the dynamic CE table.

10/20/2022    SCIEX OS

How To View Metric Plots in the Analytics Mode in SCIEX OS Software

To view trends (such as internal standard peak area) over a range of samples in a results table, a metric plot can be created in the Analytics mode of SCIEX OS software by selecting the column with the values of interest and then selecting Create metric plot from the More menu.

10/14/2022    SCIEX OS

How Can I Set the Integration Algorithm in the SCIEX OS Software?

To set the integration algorithm prior to processing quantitation data, select the Project Default Settings option in the Projects menu in the SCIEX OS software under the Analytics tile.

10/14/2022    SCIEX OS

Will the SCIEX OS Software Central Administrator Console (CAC) Run on Windows Server 2019 in a Virtual Environment?

Yes, the SCIEX OS Software Central Administrator Console (CAC) software installed on a Windows Server 2019 will run in a virtual environment and is officially supported.

10/12/2022    Pharma CRO | SCIEX OS

Custom Calculations or Flagging Rules Not Working after SCIEX OS Software Upgrade or Reinstall

After a reinstall or upgrade of SCIEX OS software, the custom calculations and flagging rules of a processing method may not work properly. A config file for the software will need to be edited to restore functionality.

09/30/2022    SCIEX OS

How Do I Open a Port in Windows Firewall for the Central Administrator Console (CAC) Software?

This article describes how to open the port for the Central Administrator Console (CAC) server when Windows Defender is used as the Windows firewall software.

09/30/2022    Pharma CRO | SCIEX OS

Data Not Visible for New Results Table Processing in SCIEX OS Software

To create a new results table in SCIEX OS software, users must browse to data sets (.wiff files) from the Analytics mode through the Results menu. When New and then Browse are selected to start a new results table, no data set is seen when the data folder is selected in the Select Folder menu. It appears as if the raw .wiff files are missing.

09/30/2022    SCIEX OS

Where Do I Put Query Files Used with Reporter Templates for MultiQuant™ or SCIEX OS Software?

Queries are used by report templates to process specific data from results tables in either MultiQuant™ or SCIEX OS Software. For a report template to utilize a query, the query file must be placed in the same directory as the report template.

09/30/2022    SCIEX OS | MultiQuant Software

Do I Have the Central Administrator Console (CAC) Server Installed for SCIEX OS Software on My PC?

Users can determine if the Central Administrator Console (CAC) server is installed on their PC by checking if the splash screen at startup displays the name SCIEX OS Central Administrator Console or by checking the About menu in the Configuration mode of the software. Also, one of the four workspace tiles on the home screen says Central Administration once the software loads.

09/30/2022    Pharma CRO | SCIEX OS

Is the Settings File for the Central Administrator Console (CAC) Software Forward Compatible?

Yes, the Central Administrator Console (CAC) software settings are forward compatible with future versions of the CAC for SCIEX OS software. This means that the setting files (.eac files) from older versions of the CAC software can be imported into newer CAC versions (when they become available), and all the information regarding users, workstations, projects and workgroups will be able to imported into a new version or new installation of the CAC software.

09/30/2022    Pharma CRO | SCIEX OS

Can I Install the Central Administrator Console (CAC) Software on a Server Also Running a FlexNet Licensing Server?

Yes, the SCIEX OS software Central Administrator Console (CAC) software can reside on the same server as the FlexNet Licensing Server.

09/20/2022    Pharma CRO | SCIEX OS

Is a Special Administrator Account Required for the Central Administrator Console (CAC) Software?

A special administrator account is not required for the Central Administrator Console (CAC) software. The CAC server uses the standard SCIEX OS software roles to manage CAC administration permissions.

09/20/2022    Pharma CRO | SCIEX OS

What Are the Hardware Specifications for the Central Administrator Console for SCIEX OS Software?

The hardware recommendations for the Central Administrator Console for SCIEX OS software are for a PC with a minimum of an i5 quad core, a 3 GHz processor or above, a minimum of 20 GB hard drive space, and a minimum of 8 GB RAM.

09/16/2022    Pharma CRO | SCIEX OS

Where is the Database for the Central Administrator Console (CAC) Software Stored?

Central Administrator Console (CAC) software is used to create and manage projects and users for laboratories using SCIEX OS software on multiple workstations and instruments. The CAC software database is stored in the following location: C:\ProgramData\SCIEX\Clearcore2.Acquisition\CacServerDataBackup

09/16/2022    Pharma CRO | SCIEX OS

How Does a Client PC Connect to the Central Administrator Console (CAC) Server?

The Central Administrator Console (CAC) software client connects to the CAC server using TCP ports 63333 and 44144. Users need to open the Windows firewall port when Windows Defender is used as the default firewall software. If different firewall software is used, then users must follow instructions specific to that software to open these ports.

09/16/2022    Pharma CRO | SCIEX OS

How Can I Install the Free Extractables & Leachables Library in SCIEX OS Software?

This article describes how to download and install the free Extractables & Leachables library into SCIEX OS software to test a library matching workflow.

09/16/2022    SCIEX OS

What Happens to the SCIEX OS Software when the License for the Central Administrator Console (CAC) Software Expires?

If the license expires for the Central Administrator Console (CAC) software, the server data is maintained in the CAC database, but users won’t be able to launch the CAC application. The SCIEX OS software installed on the client workstations will continue to launch and work with last retrieved data while the CAC server is not reachable.

09/16/2022    Pharma CRO | SCIEX OS

Internal Standard Recovery Calculations in MultiQuant™ and SCIEX OS Software

SCIEX OS and MultiQuant™ software both calculate internal standard (IS) recovery but use different approaches. SCIEX OS software uses a built-in functionality for creating custom formulas to calculate IS recovery. MultiQuant™ software requires a query to be applied to the results table to perform the IS recovery calculation and to add a custom IS recovery column to the results table.

09/16/2022    SCIEX OS | MultiQuant Software

How to Export the Settings for the Central Administration Console (CAC) Software

Users can export the security settings from a Central Administration Console (CAC) server used to manage SCIEX OS software users and projects. The settings are exported as an ECAC file and can be imported into another CAC server installation to transfer the security settings. 

09/16/2022    Pharma CRO | SCIEX OS

Valve Model Will Not Stop Equilibrating In SCIEX OS Software 2.1

When equilibrating any MS method on a SCIEX 7500 system, the "Valve Model" will never finish equilibrating even after the specified time when using SCIEX OS software 2.1.

09/14/2022    SCIEX OS | Triple Quad Systems

Unable to Export IDA Table From SCIEX OS Software into Excel

When viewing IDA data in the Explorer mode of SCIEX OS software, the IDA table data will not export into Excel. Exporting the data directly to Excel is not supported in SCIEX OS software, and the best workaround is to select the top row of the table and select Ctrl, Shift, End to copy the IDA data directly to Excel.

07/23/2022    SCIEX OS

Download a Free Ion Library for LibraryView™ Software

A free-of-charge ion library for LibraryView™ software is available from the SCIEX Software Downloads page. No license is required to use the Accurate Mass E&L Open Access HR-MS/MS Spectral Library, the so-called Extractables & leachables library.

06/22/2022    SCIEX OS | LibraryView Software

What Happens During Q0 Dissociation when "Simple" or "Enhanced" Options Are Used in SCIEX OS Software?

Declustering is achieved with the SCIEX Triple Quad 7500 system using a voltage differential between the QJet® ion guide and the IQ0 lens or a voltage differential between IQ0 and Q0 (the quadrupole ion guide before Q1). This article explains how Q0 dissociation is achieved using simple or enhanced modes in the SCIEX OS software and how the D Jet™ ion guide is involved in enhanced-mode dissociation.

06/22/2022    SCIEX OS | Triple Quad Systems

Can I Use the SWATH® Variable Window Calculator 1.2 with All SCIEX QTOF Systems?

The SWATH® Variable Window Calculator 1.2 is a new updated calculator that supports all SCIEX QTOF instruments with Analyst® TF and SCIEX OS software versions.

How to Report Regression Parameters in a Results Table Created in SCIEX OS Software

Regression parameters values (e.g., r squared, slope, linear coefficient) can now be added to a results table created in SCIEX OS software from the calculated columns that are saved under the processing method.

05/18/2022    SCIEX OS

How to Export and Import Processing Methods in SCIEX OS Software

Processing methods can be exported or imported in the Analytics workspace of SCIEX OS software.

05/04/2022    SCIEX OS

Creating a Hardware Profile in Simulation Mode in SCIEX OS Software

To create a hardware profile in simulation mode in SCIEX OS software, select Simulate Device under the Settings tab when adding instruments to a hardware profile in the Configuration mode.

05/03/2022    SCIEX OS

Setting Up Manual Integration (Percentage Rule) in SCIEX OS Software

To set up the parameter Manual Integration (Percentage Rule) in SCIEX OS software, a results table and peak review will need to be open in Analytics mode. In the peak review pane, select the Peak review display settings button under the Options menu. Then select the Appearance tab. The manual integration percent rule can be set by clicking the drop down arrow as shown below.

How to Create an Ion Ratio Flag in a Report Template

To flag an ion ratio as pass or fail in a report, an If/Else statement needs to be added using the MQ_Ion_Ratio tag.

05/03/2022    SCIEX OS | Analyst Software

How to Remove "No Data" from Report Output Generated from SCIEX OS Software

An IF/ELSE statement can be created to filter out the “No data” response in a report in SCIEX OS software.

04/04/2022    SCIEX OS

How is the GETSTAT Function Used in SCIEX OS Software?

The calculated columns for a processing method in SCIEX OS software allow users to add a formula that pulls the statistics pane calculations into the results table as a custom column. This can be done using the GETSTAT function.

04/01/2022    SCIEX OS

Where Are Acquisition Method Files (Both LC and MS) Saved for SCIEX OS Software Projects?

Each SCIEX OS software project has its own, separate MS and LC acquisition method files. This article describes where the method files are typically stored.

02/25/2022    SCIEX OS

Where Is Guided Optimization Found in the SCIEX OS Software?

The SCIEX OS software offers various guided optimization methods in the MS Method mode.

02/18/2022    SCIEX OS

Can a WIFF or WIFF2 File from SCIEX OS Software Be Split into Subset Data Files?

There is currently no script or software that allows for splitting a multi-sample data file from SCIEX OS software (i.e., .wiff or .wiff2) into individual subset files.

02/18/2022    SCIEX OS

How Can I Export Results Table Data in Encrypted Text Files in SCIEX OS Software?

Users with the administrator role have access to the project settings for secure export in the Analytics workspace under the Projects drop down menu in the SCIEX OS software.

02/11/2022    SCIEX OS

Building a Quant Method in SCIEX OS Software for DAD Data

Peaks from DAD or UV chromatograms that were acquired during an LC/MS experiment can be quantified in SCIEX OS software. Open a new quantitation method in SCIEX OS software, and then select the representative sample. On the Components tab, select the drop down menu in the Experiment Index column. Select the DAD experiment for each transition and add the desired wavelength to quantitate in the Start - Stop (Wavelength) column.

12/15/2021    SCIEX OS

Is It Possible to View Extracted Ions in Separate Panes in SCIEX OS Software?

To show extracted ions in SCIEX OS software, the data file must be opened in the Explore module and then Ctrl+E is used to extract the ions of interest. The Split Traces into Separate Panes option in the Graph menu allows for the extracted ions to be displayed in separate panes.

12/15/2021    SCIEX OS

How to Print an XPS Report after Running MS Tune in SCIEX OS Software

To print the XPS report from the most recent or a prior tuning in SCIEX OS software, navigate to this location: D:\SCIEX OS Data\Optimization and then print the file report.xps.

12/15/2021    SCIEX OS

Password Guidance for Secure Network Account or Special Acquisition Account for SCIEX OS or Analyst® Software

This article provides some guidance for using a secure network account (SNA) with SCIEX OS software or a special acquisition account (SAA) with Analyst® software.

12/13/2021    SCIEX OS | Analyst Software

Generation of a New Root Directory for SCIEX OS Software Called C:\ClearCore Data

Some users have reported the appearance of a new root directory created by SCIEX OS software that is not on the D: partition where the root directory (D:\SCIEX OS DATA) is typically located, but rather on the C: drive. This C: drive root directory has a different name (C: \Clearcore Data) and does not match the root directory that appears in the Configuration mode of SCIEX OS software in the Projects menu.

12/13/2021    SCIEX OS

Mapped Network Drive Issue when Using SCIEX OS Software Version 2.1.6

Many customers reported issues when using a mapped network drive to store for data for SCIEX OS software version 2.1.6; however, if the data was download to a local drive, no errors were received.

12/07/2021    SCIEX OS

How to Check if Unknown Samples Are Within Calibration Curve Range in SCIEX OS Software

To check if unknown samples are within the calibration curve range in a SCIEX OS software results table, additional calculated columns will need to be added to the results table.

12/05/2021    SCIEX OS

How Do I Select a Library and Perform a Library Search in SCIEX OS Software?

To select a library to use with a targeted or non-targeted screening method in SCIEX OS software, open an existing processing method or edit an embedded method from a results table. Ensure that the checkbox for Quantitation and targeted identification or for Non-targeted screening is selected. Then navigate to the Library Search option to set the library search parameters.

12/05/2021    SCIEX OS

What is the Signal-to-Noise(S/N) Integration Threshold in SCIEX OS Software?

A signal-to-noise (S/N) integration threshold was introduced in SCIEX OS software version 2.1.6, which allows users to select peaks for integratin based on the S/N ratio.

12/05/2021    SCIEX OS

How Can I Sum Vitamin D2 and D3 in SCIEX OS Software?

To add the calculated concentrations for vitamin D2 and D3 together and calculate the total vitamin D, a custom calculation in SCIEX OS software will need to be created in the processing method in the Analytics mode. The calculation will add custom column named Total VitD to the results table and provide a sum of the values for the calculated concentrations for vitamin D2 and D3.

12/05/2021    SCIEX OS

Reporter Error: Cannot Retrieve Analyte: the Specific Index ("-1") Is Out of Range

If the following error is received when creating a report for a results table in MultiQuant™ or SCIEX OS software "Cannot retrieve analyte: the specific index ("-1") is out of range", ensure that the individual tags within the loop are for the same sample type (e.g., use internal standard tags if For Each Internal Standard is used for the loop).

11/22/2021    SCIEX OS

Reporter Template Suite Software: The Summary Table Always Starts on the Second Page

When a report is generated from a results table in MultiQuant™, Analyst® or SCIEX OS software, the summary table will only start appearing on the second page of the report, and the first page is left blank after the header. To resolve this issue, open the report template and select and highlight the summary table. In the Microsoft Word Layout menu, select Paragraph. De-select the option Keep with next.

11/14/2021    SCIEX OS | MultiQuant Software

Adding a Custom Column to a Results Table in SCIEX OS Software

To add a custom column to a results table in SCIEX OS Software, select Add custom column from the More menu. Provide a name for the custom column and then select OK.

11/14/2021    SCIEX OS

Reporter Template Suite Software: How Do I Get Rid of "No Data" in Report if Sample Comment Is Empty?

When using the Reporter Template Suite Software with MultiQuant™, Analyst® or SCIEX OS software, the report output may indicate "no data" in the sample comment column if there is no text in the corresponding column in the results table. To eliminate "no data" as a response in the report, try using the following IF/ELSE statement: IF EMPTY($Sample_Comment) (Text Field: none) ELSE (Text Field: Sample Comment)

How to Export Results for Specific Analytes from SCIEX OS Software

To report only the results for specific analytes from SCIEX OS Software, a results table will first need to be opened in the Analytics mode. Start by selecting one of the analytes of interest from the Components and Group Tab. If multiple analytes need to be reported, then select additional analytes by holding the Ctrl key down and selecting each additional analyte with the mouse. Then in the Reporting menu, select Export Results > Export and Save Results Table.

11/10/2021    SCIEX OS

What Are the Standard Addition Columns in a SCIEX OS Software Results Table?

Sciex OS software allows users to process standard addition results by selecting the Standard Addition option in the Components tab of the processing method in the Analytics mode. What do the new standard addition column headers mean?

11/04/2021    SCIEX OS

How to Setup Standard Addition in SCIEX OS Software

If the standard addition feature is enabled in SCIEX OS software, then the results table contains new columns, including Standard Addition Accuracy that compares the Standard Addition Calculated Concentration for a sample to the Standard Addition Actual Concentration. Standard addition is enabled in the quantitation method on the Integration tab under the Options menu.

11/04/2021    SCIEX OS

Unable to Add Users to the Security Database in Analyst® Software

When Analyst® or SCIEX OS software is launched under the local admin account, an error message is received: “The current user is not added to the security database.” This error message appears because the software was originally installed under a non-admin account.

11/04/2021    SCIEX OS | Analyst Software

Custom Calculations and Flagging Rules for Carryover in SCIEX OS Software

This article contains instructions on how to set up a new processing method in SCIEX OS software that has the custom calculations and flagging rules for carryover.

11/04/2021    SCIEX OS

How Is the Full Screen Option Enabled in SCIEX OS Software?

Select the Full Screen Mode option to use SCIEX OS as the primary application. Users cannot close the software or access other software programs.

11/04/2021    SCIEX OS

Reporter Templates Cannot Be Edited Due to Missing XML Tags

When opening a template, an error was received from the Template Content Control Manager, "Error: Specified argument was out of range of valid values." To resolve this error, the Template Content Control Manager needs to be reinstalled.

Chromatograms Are Randomly Missing from the Report after Upgrading Microsoft Office

When a report is created using Sciex OS, MultiQuant™, or Analyst® software, one of the chromatograms is randomly missing. This issue is due to Microsoft Office 365 Apps for Enterprise or Business being installed, and this version of Office is not currently compatible with the current versions of SCIEX software.

Reporter Error: Template Not in Your Trusted Location

If a reporter error shows that the template is not in the trusted location when trying to save or move a report template, then the Trusted Locations list in Word must be updated to include locations where SCIEX reports and templates are edited.

MultiQuant™ or SCIEX OS Software: The Ion Ratios on the Report and Results Table Do Not Match

When a query file is used to calculate the ion ratio calculations in a MultiQuant™ or SCIEX OS software report, the values for the internal standards ion ratios in the report output will not match those in the results table. This issue is observed when the internal standards are not assigned to the analytes in the order in which they are listed on the quantitation/processing method.

IF Statement Syntax for Calculated Columns in SCIEX OS Software 2.1.6

Reports created with SCIEX OS software use templates with embedded tags. One of the commonly used tags is the IF statement, and the basic syntax for this tag is as follows: if(<condition>;<value if true>;<value if false>)

11/04/2021    SCIEX OS

Adding or Removing Samples from the SCIEX OS Software Results Table

Open a results table in the Analytics workspace in SCIEX OS Software. Select the More menu > Add samples. In the Select Samples dialog, select the required samples.

11/04/2021    SCIEX OS

Setting Up a New Project in SCIEX OS Software

Projects can be created in the Status panel by selecting the white plus sign in a circle icon next to the projects drop down menu. Selecting the button will bring up the New Project screen where the name of the New Project will be entered. Projects can also be created in the Configuration module by selecting the Projects tab.

11/04/2021    SCIEX OS

SCIEX OS Software: Unable to Upload Exported Text File to Watson LIMS

In SCIEX OS software 2.1.6, if the internal standards appear in any location in the results table, instead of being grouped together at the bottom of the analyte list, and are distributed throughout the processing method, then the exported results text file cannot be uploaded to Watson LIMS.

11/04/2021    SCIEX OS

How To Create Sub-project Folders in SCIEX OS Software

Within projects, data can be further organized in sub-folders in SCIEX OS software. This article explains how to create a sub-project using the Add Data Sub-Folders dialog in the Configuration workspace.

11/04/2021    SCIEX OS

How to Export the Peak List in SCIEX OS Software

SCIEX OS software users can save the peak list for the currently active spectrum or chromatogram to a tab-delimited text file. This file contains information such as the centroid X-value (mass or time), peak area, height, etc.

11/04/2021    SCIEX OS

How to Apply Regional Settings in SCIEX OS Software

This article describes how to have the Windows regional settings (language, time/date formatting and regional preferences) match the formatting in SCIEX OS software.

11/04/2021    SCIEX OS

How Does SCIEX OS Software Calculate the Ion Ratio for a Component?

SCIEX OS software uses the regression parameter defined for the first component in the results table to calculate the ion ratio for all of the components in the results table.

11/03/2021    SCIEX OS

Saving Consecutive Batches for the Echo® MS System to the Same Data File Impairs Peak Splitting, Automatic Processing

For data acquired with the Echo® MS system, peak splitting is performed after acquisition is complete. If a subsequent batch is acquiring data to a file while the system is splitting peaks written to that file during the previous acquisition, then a resource conflict occurs.

11/03/2021    SCIEX OS | QTOF Systems

What is the Limit of the Path Length for the SCIEX OS Software Installer to Ensure a Successful Install?

The SCIEX OS software installation folder path is limited to 118 characters or less.

11/03/2021    SCIEX OS

Configuring a SCIEX X500 QTOF System after Downgrading SCIEX OS Software 2.0 to 1.7

After re-installing SCIEX OS software 1.7, stop the ClearCore2 service and then install the Microsoft C++ Redistributable package (vc_redis*.exe) from the install folder in the SCIEX OS 2.0 installation package. This will enable user to configure an SCIEX X500 QTOF System in the Devices workspace.

11/03/2021    SCIEX OS

Why Is No Data Acquired in EAD Fragmentation Mode on a ZenoTOF 7600 System?

If EAD fragmentation is used in an acquisition method on a ZenoTOF 7600 system, then the accumulation time must be at least three times the reaction time. If it is not, then no data will be acquired. To resolve the issue, increase the accumulation time in the method in SCIEX OS software.

11/03/2021    SCIEX OS

When Do I Click the Confirm Button in SCIEX OS Software during Watson LIMS Data Transfer?

When transferring data to Watson LIMS from SCIEX OS software, the user must wait for the transfer to complete successfully before clicking Confirm in SCIEX OS software. It the user clicks Confirm before the transfer is complete, then the transfer status will show as failed.

11/03/2021    SCIEX OS

Can Wiff1 Files Generated by SCIEX OS Software 2.1 from X500 Series Instruments be Searched with ProteinPilot™ Software?

X500 series instruments generate wiff2 file formats, but with the newest version of SCIEX OS Software 2.1, they can also generate a .wiff1 file. Only .wiff1 files can be used to perform a ProteinPilot™ software search.

Does SCIEX OS Software Have Functionality To Help My lab Achieve Compliance with Regulations?

Yes. SCIEX OS software has all the key data integrity features required to reduce your regulatory risk, including configurable security, electronic signatures, and audit trails.

11/02/2021    SCIEX OS

Can a LIMS System Be Connected Directly to SCIEX OS Software?

Yes, SCIEX OS software supports the ability to send results directly to Watson LIMS. Other LIMS connections are possible through a third-party provider, Data Innovations.

11/02/2021    SCIEX OS

Can an Analyst® Software Results Table Be Opened in SCIEX OS Software?

No, an Analyst® software results table cannot be opened in SCIEX OS software. The user will have to recreate the Analyst results table in SCIEX OS software.

11/02/2021    SCIEX OS | Analyst Software

Magnifying Chromatogram Peaks Displayed in a SCIEX OS or MultiQuant™ Software Report

The chromatograms of peaks that are displayed in a report generated in SCIEX OS or MultiQuant™ software will retain the format of the original peaks displayed in the peak review pane. To magnify the peaks displayed in a report, the zoom settings will need to be changed in the peak review settings for MultiQuant or SCIEX OS software.

11/02/2021    SCIEX OS | MultiQuant Software

What Is the Peak Split Algorithm when Using a Marker Peak with the Echo® MS System?

The marker well in the Echo® MS System is defined by the user in the acquisition batch to generate a special barcode pattern at the beginning and end of the plate run. The barcode is a pattern composed of a narrow peak, then a pause, a wide peak, then a pause, followed by a narrow peak.

11/02/2021    SCIEX OS

How Is the New SCIEX OS Software Quantification Algorithm Better than the Original Analyst® Software Classic Algorithm?

The quantification algorithms for finding and integrating peaks in SCIEX software programs have improved significantly since the Analyst® software classic algorithm was introduced. Users of the SCIEX OS software will see two main advantages when integrating peaks with the new AutoPeak algorithm.

10/07/2021    SCIEX OS | Analyst Software

How to Use the MAM Script in BioPharmaView™ Software 3.0.2 to Transfer Peptide Information to SCIEX OS Software

BioPharmaView™ software 3.0.2 has improved its multiple attributes monitoring (MAM) workflow, and the software now includes a script, MAM-BPV to Analytics Converter, used to create a quantitative processing method for the Analytics mode of SCIEX OS software.

Location of the Current Instrument Data File for SCIEX Triple Quad™ or QTRAP® LC-MS/MS 7500 Systems

The current instrument data file (current.dat file) for Triple Quad™ or QTRAP® LC-MS/MS 7500 Systems is backed up to a SCIEX OS Software folder located at C:\ProgramData\SCIEX\MassSpec\InstrumentData\YYYYMMDDHHMMSS.

Reporting Values for Calculated Concentration that Are Not Equal to N/A from a Results Table

To filter results table data in a report based on the calculated concentration not equal to N/A, add the if statement $Calculated_Concentration<>"N/A".

Creating Reports where Internal Standard Peaks Appear Normalized to the Analyte Peak in Chromatograms

When viewing an analyte's chromatogram in a report, the internal standard peak that is overlaid with the analyte's is not normalized in the report's chromatogram and part of the peak appears out-of-bounds in the peak review image. If you see an internal standard peak in the peak review pane of MultiQuant™ Software or SCIEX OS Software that is not normalized to the analyte's peak size, double click on the x-axis of that particular chromatogram.

12/04/2020    SCIEX OS | MultiQuant Software

Displaying Analytes Horizontally and Samples Vertically on a Report

To create a report where the analytes appear horizontally (as column headers) and sample names appear vertically (at the start of a row) in a report, the For Each Analyte tag will need to be nested within the For Each Sample tag.

Using an Older Version to Open a Processing Method Created in a Newer Version of SCIEX OS Software

The files created in SCIEX OS Software are not backwards compatible with other versions of the software. An error will appear when a processing method created with a higher version of SCIEX OS Software (e.g., 2.0) is opened with a previous version of SCIEX OS Software (e.g., 1.7). To open a processing method created in the latest version of SCIEX OS Software with the older version, export the processing method as a text file.

12/02/2020    SCIEX OS

Where Is the Executable File for Excel Saved on My Computer?

The executable file for Excel – Excel.exe – is located in the installation directory for the 64-bit version of Microsoft Office 365 at C:\Program Files\Microsoft Office\root\Office16. 

12/02/2020    SCIEX OS | MultiQuant Software

How to Sum the Concentrations of Isomers in SCIEX OS Software

To add the concentrations of different isomers together when processing data in SCIEX OS Software, open a processing method in the Analytics mode of the software. Select the Calculated Columns tab in the processing method and then select Add Formula to create a custom calculation for adding the isomers.  

12/02/2020    SCIEX OS

How to Open a Reporter Query Used with SCIEX OS or MultiQuant Software Report Templates

This article discusses the three ways to open a query (a file with the extension .query), a file linked to a report template that can be used to perform more complicated calculations or sorting functions in a results table.

Why Do the Results for Some Analytes Shift to a New Column in a CSV Report?

When reporting results table values to a comma-separated values (CSV) report, for some analytes, the reported information is shifted from the correct column to one column over to the left. This column-shifting issue is observed when an analyte name contains a comma.

Filtering Audit Trail Events Using a Set of Specified Criteria in SCIEX OS Software

The audited events in the audit trail can be filtered using a set of specified criteria in SCIEX OS Software by selecting the Filter icon in the upper task bar on the Audit Trail screen.

11/06/2020    SCIEX OS

How Can Audit Trail Records Be Viewed from SCIEX OS Software?

To view audit trail records in Sciex OS Software, open the Audit Trail workspace. The Audit Trial screen will appear. Select Workstation in the left pane to view the audit trail for the workstation. Select Project in the left pane to view the audit trail for the project of interest.

11/06/2020    SCIEX OS

Flags or Custom Results Are Not Showing Correctly on a SCIEX OS Software Report

A mismatch of index can occur when internal standard values from a SCIEX OS software results table are processed within a query, causing the text for the reported values to appear bolded and in red, even when there is no reason for flagging these values in the report itself. This phenomena is observed for results tables created in versions of SCIEX OS Software 1.6.1 or lower and has been fixed in versions of SCIEX OS software 1.7 or greater.

11/05/2020    SCIEX OS

Is There an Equivalent to Single User Mode in SCIEX OS Software 2.0?

Single user mode is a security mode in Analyst® software that allows for any user that is logged in to a PC using any Windows account to access the software as an administrator. However, there is not currently an equivalent to single user mode in the SCIEX OS software. The security mode options in SCIEX OS software are limited to integrated mode and mixed mode.

11/04/2020    SCIEX OS | Analyst Software

How to Remove the Extra Characters from an Analyte Name in a Report

To report the analyte name in a report without additional characters showing after the name, use the TRIM and LEN functions in the expression written in the Math Field Type tag in the report template.

11/04/2020    SCIEX OS | MultiQuant Software

Controlled Ways to Output Data from SCIEX OS Software

The controlled ways to output data from the SCIEX OS software Analytics workspace include 1) exporting a results table; 2) Transferring  the results table data to a LIMS; and 3) Creating a report though the Reporting tab option Create Report and Save Results Table.

11/04/2020    SCIEX OS

Adding a Flag or a Custom Flag Created in SCIEX OS Software to a Report

After creating a new results table in SCIEX OS software, add the Outlier Reasons column in the quantitation method to the Input tab of a query. Then, in the AnalystCalculations tab of the query, use the search function to find the flag of interest. Have the Outlier Reasons column added to the query's Output tab, and then link the Outlier Reasons column to the report template using a custom tag.

11/04/2020    SCIEX OS

Initiate Transfer to Watson LIMS in SCIEX OS Software Prevents Analyst® Software from Launching

When the feature Initiate Transfer to Watson LIMS is selected in SCIEX OS software, software users find that the Analyst® software installed on the same workstation will no longer launch. This is the expected behavior for Analyst software and depends on the configuration and the installation order of other SCIEX software that were installed on the same PC. 

10/30/2020    SCIEX OS | Analyst Software

Adding Custom Columns to a SCIEX OS Software Report Template

In SCIEX OS software, a custom column added to a results table can be included in a report template with a custom tag.

10/30/2020    SCIEX OS

Watson LIMS Needs To Be in Compatibility Mode for Use with SCIEX OS Software

When transferring data from SCIEX OS software to Watson LIMS, the Watson software did not appear to be importing the data.

10/29/2020    SCIEX OS

Where Are the Event Logs for SCIEX OS Software Archived?

When event logs in SCIEX OS software become too large, the software will create an archive event log file. This file is located in the directory C:\ProgramData\SCIEX\Clearcore2.Acquisition.

10/29/2020    SCIEX OS

Where Can I Find My Hardware Profile in SCIEX OS Software?

The hardware profiles for instruments supported by SCIEX OS software are stored in a file called HardwareProfile.hwp that is located in the following directory: C:\ProgramData\SCIEX\Clearcore2.Acquisition.

10/29/2020    SCIEX OS

Where is the SCIEX OS Software Security Database Found?

The SCIEX OS software security database files are located at C:\ProgramData\SCIEX\Clearcore2.Acquisition, and the file is called Security.data.

10/29/2020    SCIEX OS

How to Export a Statistics Table From SCIEX OS Software to Excel

The statistics pane can be accessed from the Views menu in the Analytics module of SCIEX OS software. After opening the results table, select Statistics pane from the Views menu and a new table appears below the results table. To export the contents of the statistics table from SCIEX OS, the table can be copied to Excel by highlighting all rows, and then selecting Ctrl+C. In an open Excel spreadsheet, the selected columns and rows can be pasted using Ctrl+V.

10/28/2020    SCIEX OS

What Is the SCIEX OS Software Equivalent to the Area Threshold Feature in Analyst® Software?

SCIEX OS software does not have a field for the Area Threshold as found in the Analyst® software quantitation method wizard that allows users to set the criteria for peaks to appear as "not found" in a results table. Instead, a custom flagging rule must be created in SCIEX OS software to enable this type of filtering for peak area.

10/22/2020    SCIEX OS

Can Regular Expressions (Regexes) Used for Data Filtering in SCIEX OS Software Be Saved?

To export and import filter settings so they can be used across multiple results tables and projects in SCIEX OS software, a regular expression (regex) can be created using a custom filter in an existing results table. The custom settings can be exported as a .cset file using the Table Display Settings export feature and reapplied to another table using the Import feature.

10/22/2020    SCIEX OS

Incorrect Argument Error when Adding Standards to a Results Table in SCIEX OS Software

When adding multiple standard samples with the same name to a results table in SCIEX OS software, an error was generated: "Incorrect Argument. An item with the same key has already been added." This article explains the workflow that generates this error and provides a workaround.

10/22/2020    SCIEX OS

Differences between “Find Any” and “Find” in FormulaFinder in SCIEX OS Software

In SCIEX OS software, the “Find Any” function in Formula Finder considers just the monoisotopic peak to be within the m/z tolerance. Both odd (in italics) and even electron elemental compositions are provided. When using “Find Any” element ranges “From” and “To” are considered. The “Find” function in Formula Finder uses all constraints, including the mass tolerance for all selected peaks in the isotope pattern and the settings from the Options dialog.

10/22/2020    SCIEX OS

Can I Use a Regular Expression (Regex) to Filter Data in SCIEX OS Software?

A regular expression (regex) can be used to filter data in a results tables and in reports created with SCIEX OS software.

10/22/2020    SCIEX OS

SQLite Exception Error in the Regulated Version of SCIEX OS Software

While setting up the regulated version of SCIEX OS software (CFR features activated), the following error message appeared when accessing the Audit Map portion of SCIEX OS software: "System.Data.SQLite.SQLiteException : unable to open database file." To resolve this issue, change the Windows permissions so that the user has full access to Audit Trail files.

10/20/2020    SCIEX OS

C Drive Free Space Requirements when Using SCIEX OS Software

SCIEX OS software performs best when 20 GB of free space is available on the C drive (C:). Furthermore, SCIEX OS software creates log files in order to monitor both instrument (on an acquisition PC) and software (on both acquisition and processing PC's) performance. These log files incremently increase and can accumulate to a total size of 35 GB. This sets a limit of 55 GB of space needed on the C drive to use SCIEX OS software optimally.

05/14/2020    SCIEX OS

Printing an Unsecured Report as a PDF with MultQuant™ or SCIEX OS Software

Reports printed as PDF files from either SCIEX OS or MultiQuant™ software are always secure because they are printed using pdfFactory. A workaround must be used to print unsecured PDF reports.

05/13/2020    SCIEX OS | MultiQuant Software

What Are the Most Common Installation Issues for SCIEX OS Software 1.7?

Some of the most commonly observed installation issues with SCIEX OS software 1.7 are summarized here. Instructions on how to uninstall previous versions of SCIEX OS software are also included.

04/28/2020    SCIEX OS

Which Version of Watson LIMS Is Compatible with SCIEX OS Software 1.7?

SCIEX OS software 1.7 has been tested with Watson LIMS server/client version 7.6, Oracle server/client 12.2.0.1.0 SE (64-bit) and 12.2.0.1.0 (32-bit).

04/16/2020    SCIEX OS

How to Export UV Data into a Text File in SCIEX OS Software

Open a data file that also contains UV data in SCIEX OS software in the Explorer module. Locate the Show menu and select UV/DAD/ADC Channel. Once UV trace is displayed, click Data and Peaks Table under the Show menu to display the table. Export the data by right clicking on the table and selecting Export Data as Text from the menu.

04/14/2020    SCIEX OS

How To Create an Unsecured PDF Report in Sciex OS Software

In order to create reports in SCIEX OS software in the portable document format (PDF) that are unsecured, the PDF setting in the SCIEX OS config file will need to be updated. Please follow below instructions to edit the config file.

03/20/2020    SCIEX OS

How to Add Select Samples to a Report Created in SCIEX OS Software

To add only selected samples from a results table created in SCIEX OS software in a report, uncheck the Reportable column for the sample you do not want to report. Then edit your report template to include an If statement within a ForEachSample loop to include $Reportable="TRUE".

03/20/2020    SCIEX OS

Sciex OS Software Report Error: An Item with the Same Key Has Already Been Added

An error was received while using Sciex OS software to generate a report: "Error during Report generation. An item with the same key has already been added." No report was generated. To resolve this issue, open the report template and see if a query file is linked through the Meta Field tag. If a query is linked, open the query and evaluate it for any duplicate column names. Delete any duplicate columns from the input sheet.

03/20/2020    SCIEX OS

Which Folders Should Be Backed Up Before Upgrading the SCIEX OS Software?

Prior to upgrading or removing SCIEX OS software, back up the SCIEX OS Data folder and the SCIEX folder in the Program Data folder.

03/20/2020    SCIEX OS

Using an External Calibration Curve in SCIEX OS-MQ Software

To use an external calibration curve with a results table created in SCIEX OS-MQ software, use the Export calibration (and save results...) feature in the Options menu after the calibration curve has been generated.

03/17/2020    SCIEX OS

How To Enable the New Intact Protein Quant Feature in SCIEX OS 1.7 Software

The Intact Quant feature in Sciex OS 1.7 software platform enables the quantitative processing of intact proteins without sample prep and allows for the complete and compliant intact reconstruction of protein data sets. To activate this feature, an additional, add-on software license, SCIEX OS IntactQuant 1.7 (part number 5078597), must be installed in the C:\ Program Files\SCIEX\SCIEX OS folder.

03/17/2020    Academia Omics | Pharma CRO | SCIEX OS

Common Project Area Folder Write Access Error in SCIEX OS Software

When creating a new project in SCIEX OS software, the following error was received: The current user does not have write access to the requested project. This issue was resolved by copying the common-project-area folder in the SCIEX OS Data folder over from a different PC with an intact root directory.

03/06/2020    SCIEX OS

Why Do the Calculated Concentration Values Show as Degenerate in SCIEX OS Software?

In SCIEX OS software, if a value in the Calculated Concentration column displays as degenerate, this is because the values for Actual Concentration for standard samples have not been added to the results table.

12/16/2019    SCIEX OS | MultiQuant Software

Reporter 3.2: Including Only the Results for the Quantifier Ion in a Report

To include only the data associated with the quantifier ion in a report, group the quantifier and qualifier ion for a particular analyte, and then use the For Each AnalyteGroup loop around the information to be reported.

SCIEX OS Software: How to Choose the Format for a Numerical Field in a Report

By default, the format for a numerical field in a report is taken from the results table itself if the format is not set in the report template tag. If a specific numerical format is desired for a particular field in a report, certain default formats (such as the number of decimals desired or scientific notation) can be chosen in the Format field in the tag for that value. Otherwise, In the SCIEX OS software results table itself, the number format can be set for individual columns by editing the Number Format in the Results Table Display Settings options.

SCIEX OS Software: Internal Standard Data in Report Does Not Match the Results Table

If a report generated from SCIEX OS software using a template and a query contains information about internal standard samples, the internal standard information showing in the results table does not appear correctly in the report. To resolve this issue, all internal standard samples must be listed at the bottom of the SCIEX OS software processing method in the components section.

11/09/2019    SCIEX OS

No Help Topics Appear in the SCIEX OS Software Help System

Selecting the question mark in the upper right hand corner of SCIEX OS software launches the SCIEX OS Help System screen. If no information appears for a selected topic, the SCIEX OS Help.chm file located in the C:\Program Files\SCIEX\SCIEX OS\Documentation folder needs to be unblocked.

11/09/2019    SCIEX OS

Modifying a Report Template to Report Select Sample Types Using IF Statements

An IF statement can be added to limit the sample types that need to be output to a report from a results table created in Analyst® software, MultiQuant™ software or SCIEX OS software. Below are few examples of how to write an IF statement within the loops ForEachSample or ForEachAnalyte.

Where are the SCIEX OS Software Report Templates Located on My PC?

If a report template needs to be opened for editing or if a new custom template needs to be added to the template list in SCIEX OS software, then navigate to C:\ProgramData\SCIEX\Analytics\Reporter to locate or add a template.

11/08/2019    SCIEX OS

Direct LIMS Transfer in SCIEX OS Software Does Not Require a Template

Transferring data to a LIMS system from a results table created in SCIEX OS software does not require a template. Once the results table is saved and locked, under the Reporting menu, select Transfer results to LIMS, and two LIMS export options will appear in the LIMS transfer dialog box.

11/07/2019    SCIEX OS

Can the Loops ForEachSample and ForEachVisibleSample Be Used in the Same SCIEX OS Software Report Template?

No, the loops, ForEachSample and ForEachVisibleSample, cannot be used within the same SCIEX OS software report template. Similarly, ForEachAnalyte and ForEachVisibleAnalyte cannot be used together in the same report template.

11/01/2019    SCIEX OS

Problem Uninstalling SCIEX OS Software Stems from Hidden SQL Driver

While removing the SQL 2008 R2 Setup program during a SCIEX OS software de-installation, an error was received—indicating that the issue was due to the 2008 R2 RsFx driver, a "hidden" program that needs to be manually uninstalled using either the command prompt or regedit Windows functionalities.

10/29/2019    SCIEX OS

How to Update or Link a Query File to a Report Template

If the name of a query is ever changed or updated or if a new query needs to be attached to a report template, the query must be relinked to the template used for reports created in MultiQuant™, Analyst® or SCIEX OS software.

What Integration Algorithms Are Used by SCIEX OS Software?

The SCIEX OS software has three integration algorithms that can be used to integrate peaks, and these options are listed in the Project Default Settings in the Analytics module: MQ4, AutoPeak and Summation.

10/22/2019    SCIEX OS

Resolving the Report Error in SCIEX OS Software: "Error during Report generation"

This is a known issue in the ProteinPilot™ software 5.0.2 and was resolved with a patch.

10/22/2019    SCIEX OS

Report Template Editing Blocked after Upgrading SCIEX OS 1.6.1 Software from a Previous Version

After updating SCIEX OS software from version 1.5 to 1.6.1, report template may be unable to be edited if Analyst® software is installed on the same PC. An error message appears in the Microsoft Word report template document: "Reporter Office addin. The provider with the name Quantitation already added to content control addin.Multiple custom type XML files found for the same provider." This article explains how to resolve this issue.

10/22/2019    SCIEX OS

Changing the Integration Algorithm in SCIEX OS 1.6.1 Software

To change the integration parameter used in a quantitative processing method in the Analytics module of SCIEX OS Software, select the Projects menu, and under the Projects default settings, select Quantitative Processing > Integration Defaults > Integration Algorithm.

10/17/2019    SCIEX OS

What File Format Can Report Templates Be Created In?

Report templates—used to create reports from results tables generated in Sciex OS, MultiQuant™ and Analyst® software—must always be in the format of a Word document with a file extension .docx. However, the output report itself can be created in several formats including Word, HTML, PDF, and CSV.

How to Access the SCIEX OS Software Help Menu

To access the SCIEX OS software help menu, click on the question mark in the upper, right-hand corner.

10/16/2019    SCIEX OS

Ensuring the Positive Hits Report Includes Only Positive Hits from a Results Table Generated in SCIEX OS Software

To ensure that only the positive hits appear in a report generated using the Positive Hits template rather than all visible results in a results table, the results table itself first needs to be filtered to show only positives prior to running the report.

10/16/2019    SCIEX OS

Data for Custom Calculations Show as Not Calculated on a Report

In reports created from results tables in Analyst® and MultiQuant™ software, queries are used to compute the values for custom calculations, but occasionally the data appears as "Not calculated" on the report. This issue appears when the query file linked to the report template is not saved to the same folder as the report template.

Centering a Peak in a SCIEX OS Software Peak Review Pane

To adjust the location of the peak in the window and to center it, select the peak review icon in the results table, click Options and then Peak review display settings. In the Zooming tab, select the Zoom time axis to view peak check box and add the desired retention time.

10/11/2019    SCIEX OS

To Make Reporter Tags Visible in a Report Template Unblock Them

If a report template is emailed or transferred to another computer and then opened, the xml tags may not be visible because they were blocked for security reasons.

Unlocking the Secure PDF Files Created by SCIEX OS Software

To unlock a secure PDF file created using SCIEX OS software reporter, open the document in a web browser and then print the newly opened document using either Microsoft Print to PDF or Save As PDF.

10/04/2019    SCIEX OS

Migrating an Analyst® Software Compound Library to Sciex OS Software

To migrate compounds in an Analyst® software library to Sciex OS software, navigate to the Library module in Sciex OS software, and select Analyst Compound Library in the import options. The imported library must be named. Instructions for naming the library are included here.

How Are Analytes Deleted from a Processing Method in Sciex OS Software?

To delete unwanted analytes from a processing method in Sciex OS software, highlight the rows, right click and select Delete Rows.

10/03/2019    SCIEX OS

Entering the Formula for a Stable Isotope Labeled Compound in SCIEX OS Software

To enter the formula for a stable-isotope-labeled compound in the Components tab of a processing method in SCIEX OS software, some specific rules must be followed. When entering the formula, the atomic mass of the isotope is added before the single letter chemical abbreviation of the element, and then the total number of atoms of each isotope follows the chemical symbol.

10/02/2019    SCIEX OS

How Can Library Hit Results Be Filtered by Score in a SCIEX OS Software Results Table?

To filter library hit results in a SCIEX OS software by library score and to remove any results without a proper score (presenting with N/A), a custom filter can be used in the Analytics mode.

10/02/2019    SCIEX OS

Exporting and Importing a Flagging Rule in SCIEX OS Software

Flagging rules for IS Recovery can be exported from an existing method in the Analytics workspace of Sciex OS software, and then the exported file can be re-imported into a new processing method using the Export/Import functions in the Flagging Rules screen.

09/29/2019    SCIEX OS

Resolving SCIEX OS Software Error: Cannot Open LibraryViewServiceHost Service on Computer

When trying to access the Analytics mode in SCIEX OS software when logged into Windows as a user without administrative privileges, an error is received: "Error has occurred: Cannot open LibraryViewServiceHost service on computer '.'. LibraryView service must be running." The LibraryViewServiceHost was not started when the Analytics mode was opened, and a workaround needs to be used to launch it.

09/27/2019    SCIEX OS

Why Is There a .Special File Placed in the SCIEX OS Software Project Folders?

SCIEX OS software creates a special placeholder file (with a file extension of .special) and places these files in the root of every known SCIEX OS project sub-folder. SCIEX OS software uses both the project folder names and the .special files to identify its known folders, and it will not recognize the folder if the .special files are removed.

09/26/2019    SCIEX OS

Can SCIEX OS and MasterView™ Software Be Installed on the Same Computer?

SCIEX OS and MasterView™ software are not compatible, and they cannot coexist on the same computer.

09/24/2019    SCIEX OS | MasterView Software

Adding Users to the SCIEX OS Software Security Database

To add users to SCIEX OS software Security Database, the user who installed the software needs to navigate to the User Management section in the Configuration mode and select the plus sign next to the User name or group drop-down menu.

09/23/2019    SCIEX OS

How Do I Generate a Report with Only the Quantifiers in SCIEX OS Software?

Open the Analytics workspace in SCIEX OS software and open a results table. In the More menu, click Table Display Settings. A list of column names will appear. Select Reportable to make this option visible. Selecting the Reportable column shows whether or not the result is included in reports, exports, and LIMS transfers. Under Reportable column, select Quantifiers for the component.

09/23/2019    SCIEX OS

Where Can I Find My Service Package for SCIEX OS 1.6.1 Software?

Any service package generated for SCIEX OS 1.6.1 software can be found as a zipped (compressed) file at C:\ServicesPackages.

08/09/2019    SCIEX OS

Adding a New Flagging Rule in the Analytics Workspace of Sciex OS Software

To add a new flagging rule in the Analytics workspace of SCIEX OS software, open the Analytics workspace. Click Process Method and select New. Then select the Flagging Rules page and click Add Rule.

08/09/2019    SCIEX OS

Sciex OS 1.5 Software Error: Processing Method Contains No Components or Is Incompatible

When opening a processing method in Sciex OS 1.5 software, an error was received, indicating that the method contained no components or was incompatible. Removing the sub-folder (C:\SCIEX OS Data\<PROJECT NAME>\Quantitation Methods\<Sub-Folder>) resulted in normal software operation.

08/09/2019    SCIEX OS

How Are the Ion Ratio Flagging Rules Set Up in SCIEX OS 1.5 Software?

To set up the ion ratio flagging rules in SCIEX OS software, select a processing method and then select edit. The rules can be changed under Flagging Rules and Ion Ratio Acceptance options under this menu.

08/08/2019    SCIEX OS

Protocol for Resolving the SCIEX OS Error: SciexLibraryService Rejected the Session-Establishment Request

Use the following protocol to change the port 8081 to resolve the software error that occurs wtih both Sciex OS and MasterView/LibraryView installations: SciexLibraryService rejected the session-establishment request.

07/24/2019    SCIEX OS

Does SCIEX OS Software Have an Equivallent Security Configuration to Analyst® Software's Mixed Mode?

In Analyst® software, there are three security configuration modes available: Single User, Integrated and Mixed. Are there functional equivalents of all three available in SCIEX OS software? In short, no, there are not.

07/14/2019    SCIEX OS | Analyst Software

Converting MultiQuant™ Software Qmethods to Sciex OS Software Qmethods

This article contains a step-by-step guide for converting a MultiQuant™ 3.0.3 software quantitation method (.qmethod) file to a Sciex OS 1.5 software quantitation method file (.qmethod).

06/26/2019    SCIEX OS | MultiQuant Software

General SQL Installation Errors for SCIEX OS and LibraryView™ Software

Installing any program with SQL-based software components (e.g., SCIEX OS and LibraryView™ software) requires that a certain rules are observed. These rules are set by Microsoft, and are not covered in the release notes for the SCIEX software installation that includes the additional of SQL components.

05/23/2019    SCIEX OS | LibraryView Software

System Error Causing SCIEX OS Software Acquisition Stoppage Resolved by Clearing Data Folder

Sample acquisition using Sciex OS 1.5 software was stopped, resulting in a system error. The drive where the SCIEX OS Data folder was stored was full, so backing up and clearing out older data allowed for data acquisition to proceed.

05/13/2019    SCIEX OS

Using the Purity Score To Assess a Match to My Library Search Results in SCIEX OS Software

All peaks from both the unknown and library spectra are used to calculate the Purity Score, which is used to assess how well a compound matches an existing library compound. A high value indicates a high probability that the unknown spectrum has been correctly identified; a low value indicates the match is less certain.

04/23/2019    SCIEX OS

Why Don't I get Hits when the Precursor Mass Tolerance Is Set to 0.01 Da when Searching SCIEX Libraries?

Searches of SCIEX libraries with the precursor mass tolerance set to 0.01 Da may not yield any hits. This is due to the fact that some of SCIEX libraries contain spectra that were acquired with inaccurate precursor m/z.

04/19/2019    SCIEX OS

Replacing the Logo Shown in My Report in SCIEX OS Software

To replace the logo shown in a report in SCIEX OS software after the results table is reviewed, click Reporting, then Create Report, and then save the Results Table. Click Replace Logo.

04/19/2019    SCIEX OS

The non-targeted peak searching algorithm determines which peaks are included for further library or formula finder searches in the Analytics mode of SCIEX OS Software, and the parameters that can be modified are described here.

04/17/2019    SCIEX OS

Installing the SCIEX OS Software CFR License

Save the CFR license file to the C:\ Program Files\Sciex\Sciex OS folder where the SCIEX OS license is located.

04/15/2019    SCIEX OS

User Role Not Assigned Correctly in SCIEX OS Software

To resolve the error "You do not have the correct SCIEX OS role permissions to access this product" in SCIEX OS software, the software needs to be opened under the Windows user login that the software was installed under.

04/05/2019    SCIEX OS

How Does the Formula Finder Algorithm Work in Sciex OS Software?

The Formula Finder algorithm in SCIEX OS software tries to predict the possible chemical formula based on the MS and MS/MS spectrum using the precursor ion's mass accuracy, isotopic pattern and MS/MS fragmentation pattern.

04/02/2019    SCIEX OS

Uninstalling and Reinstalling SCIEX OS 1.x Software

This article describes how to uninstall SCIEX OS software and manually remove certain files in order to completely remove the software.

03/21/2019    SCIEX OS

Recalibrating TOF MS or TOF MS/MS Spectra in SCIEX OS Software

To recalibrate a TOF MS or TOF MS/MS spectrum or recalibrate samples in SCIEX OS software, open the data file in Explore mode, select process, and then select Recalibrate Spectrum or Recalibrate Sample.

03/21/2019    SCIEX OS

Exporting a Centroid Spectrum from SCIEX OS Software

To export a centroid spectrum from SCIEX OS software, load a data file and open a spectrum in explore mode; click Centroid Spectrum from Process drop-down list, and then select Export from the File drop-down list.

03/01/2019    SCIEX OS

Why is the Option to Sum Multiple Ions Grayed Out in SCIEX OS Software?

In order to use the Sum Multiple Ions option in the SCIEX OS software, the group name for each compound needs to be entered into the Group column.

02/28/2019    SCIEX OS

SCIEX OS 1.5 Software Launch Errors Overcome by Starting Clearcore2 Services

The Clearcore2 Service runs in the background for SCIEX OS 1.5 software. The service will need to be started manually in the Services section of the Control Panel if SCIEX OS will not launch.

02/28/2019    SCIEX OS

Choosing the Peak Selection Criteria when Using SCIEX OS AutoPeak Integration Algorithm

This articles explains what type of peak selection criteria can be used with the SCIEX OS AutoPeak integration algorithm.

02/15/2019    SCIEX OS

Applying the Saturation Correction when Working with SCIEX OS AutoPeak Integration Algorithm

Saturation correction can correct for detector saturation effects. To make these changes in SCIEX OS software when working with the autopeak integration algorithm, select the saturation correction button in the method and specify the threshold value.

02/15/2019    SCIEX OS

Applying Results Table Column Settings to Another Results Table in SCIEX OS Software

To apply the custom result table column settings from one Sciex OS software results table to another, open a results table in the Analytics mode. Then set the custom column order that you want to be displayed by clicking the More button, and then choose Table Display Settings.

02/15/2019    SCIEX OS

What are the Library Search Algorithms in SCIEX OS Software?

When setting up a new processing method in SCIEX OS software's Analytics mode, there are three different library search algorithms that the user can select under the library search parameters dialogue: candidate, confirmation and smart confirmation search.

02/15/2019    SCIEX OS

Using the Same Peak Model for All Components in a Group with SCIEX OS AutoPeak Integration Algorithm

In order to use the same peak model for all components in a group in SCIEX OS software, the user must enable the Peak Selection by Group and Process by Group settings in the Project Default Settings before creating the processing method.

02/15/2019    SCIEX OS

SCIEX OS 1.5 Installations on Windows 7 Require a TDC4 Driver Update

SCIEX OS 1.5 installations on Windows 7 require a TDC4 driver update. Please navigate to the installation folder for Sciex OS and select the Drivers folder. Inside should be a program xTDC4_driver_v1.1.0.exe which will remove version 1.0.3 and install 1.1.0.

02/08/2019    SCIEX OS

Changing the Orienatation of Peak Review Chromatograms in a Reporter Template

If the peak review chromatograms are showing in the wrong orientation on an Analyst® or MultiQuant™software report, the page orientation for the actual Word document template may be set to portrait rather than landscape.

Recommended NIST Library Processing Method Settings

The recommended library search settings when using the NIST library with MasterView™ or SCIEX OS software are listed here and include deactivating collision energy filtering.

Sciex OS 1.3 Error "Services Cannot Launch" Due to Windows 10 Incompatibility

An error message is received when trying to launch SCIEX OS 1.3 that indicates that services cannot start. Ensure that the software is installed on a Windows 7, not Windows 10, operating system (OS). Only SCIEX OS version 1.4 and greater are compatible with Windows 10 OS.

12/27/2018    SCIEX OS

Can the Pressure Trace Be Monitored or Saved in SCIEX OS 1.4 Software?

Currently the pressure traces for many liquid chromatogram instruments can be monitored in real-time in the SCIEX OS software, but not be saved to a specific location.

10/16/2018    SCIEX OS

Can Report Templates from Analyst® or MultiQuant™ Software Be Used with SCIEX OS?

Report templates used previously with Analyst® or MultiQuant™ software should work with SCIEX OS, with the exception of MultiQuant templates that include a table query.

07/03/2018    SCIEX OS

Calculating Molecular Mass for Compounds with Isotopes in SCIEX OS

To calculate the molecular mass of compounds containing isotopes in SCIEX OS, select the Mass Calculators in the Explorer mode and then specify the chemical formula for the Mass Property tab. For isotopes, precede the chemical symbol with the weight of the isotope.

07/03/2018    SCIEX OS

Protocol for Downgrading SCIEX OS 1.4 to 1.3

If downgrading from SCIEX OS 1.4 to 1.3 is required, follow the instructions below.

07/02/2018    SCIEX OS

Installing SciexOS 1.4 on a PC where Other SCIEX Software Was Previously Installed

SciexOS 1.4 uses the LibraryView™ Framework in the background to manage libraries and library searching. Older versions of the framework may be on the PC that can interfere with the installation of 1.4. Also, older version of Microsoft SQL server and .NET Framework can cause the installation process to fail. This article contains information on steps to take to remove older versions of the aforementioned tools.

07/02/2018    SCIEX OS

Does SCIEX OS 1.4 Support Queries?

SCIEX OS 1.4 does not support table queries in the Analytics component of the software.

07/02/2018    SCIEX OS

How to Update Retention Times in Sciex OS

To update the retention time (RT) of an analyte in Sciex OS, open a results table consisting of grouped components with different RTs, and then follow the instructions provided below.

05/03/2018    SCIEX OS

Setting up a Rack Changer in Sciex OS Simulation Mode

To set up the RackChanger in Sciex OS in simulation mode, go to Devices under Configuration mode. Select Add and choose Integrated System and follow the directions below.

03/22/2018    SCIEX OS

Which Software License Is Used for LibraryView™ 1.2 Software Installed with SCIEX OS 1.3?

When installing LibraryView™ 1.2 software alongside SCIEX OS 1.3, use a LibraryView 1.0 license, PN 5022277.

12/21/2017    SCIEX OS | LibraryView Software

Opening SCIEX OS Results Tables Containing UV and MS Data

To open a SCIEX OS results table created using a data file (.wiff2) with both UV and MS data, both data types must be selected for processing when creating the quantitation method in Sciex OS. Otherwise, an error message is received "Error. Cannot be empty, Parameter name: experiments" when opening Sciex OS results tables after they are created.

12/13/2017    SCIEX OS

How Do You Obtain a Token for ChemSpider Software?

The Formula Finder Settings panel in Sciex OS software requests a ChemSpider security token after selecting the ChemSpider Service. To obtain a token, users will have to register on the ChemSpider website.

11/08/2017    SCIEX OS

Sciex OS Software Startup Always Defaults to License Activation Screen

Every time Sciex OS software is launched, the license activation screen continues to appear with all fields blank, even though previous launches of the software indicate that the software license was installed successfully. Reinstalling the software as an administrator using a local admin user will resolve this issue.

09/20/2017    SCIEX OS